![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2014009:Design
Designed by: Daria Niewiadomska Group: iGEM16_UAM_Poznan (2016-10-12)
pxylS-E1_5'UTR->sfGFP_B
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We were looking for the best method to optimize gene expression in E.coli.
Source
The xylS promoter is a modified version of xylR-xylF-xylA1 fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence.